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Anoikis is a specific form of apoptosis resulting from the loss of cellular attachment to extracellular matrix or other cells. Thus, it is difficult to simulate these conditions in vitro to generate a controlled, efficient assay to study anoikis.

 
   
   
   
   
   
   
   

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  Researchers are developing several methods to study anoikis. This is a selected list of available methods.
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  Microscale method for analysis and quantification of anoikis using micromolded, non-adhesive hydrogels.

Miniaturization of an Anoikis assay using non-adhesive micromolded hydrogels. Rago AP, Napolitano AP, Dean DM, Chai PR, Morgan JR. Cytotechnology. 2008 Feb;56(2):81-90.

 

 

 

 

 

 

 

     

 

    Using a time resolved fluorometric assay with Europium-labelled Annexin V

A novel time resolved fluorometric assay of anoikis using Europium-labelled Annexin V in cultured adherent cells. Engbers-Buijtenhuijs P, Kamphuis M, van der Sluijs Veer G, Haanen C, Poot AA, Feijen J, Vermes I.Apoptosis. 2005 Mar;10(2):429-37.

 

       
 

 

 

 

 

 

 

 

 

 

 

 

       
       
       
       
       
       
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